rabbit polyclonal anti agr2  (Novus Biologicals)

Journal: Frontiers in Oncology

Article Title: Genomic Network-Based Analysis Reveals Pancreatic Adenocarcinoma Up-Regulating Factor-Related Prognostic Markers in Cervical Carcinoma

doi: 10.3389/fonc.2018.00465

Figure Lengend Snippet: (A) Flow chart identifying the candidate PAUF-associated prognostic markers. (B) Network view of 22 genes using interactive analysis with cBioPortal network analysis ( http://cbi oportal.org). Among candidate genes, high expression of AGR2 protein and low expression of BRD7 protein are significantly associated with favorable disease-free survival while the low expression of POM121 protein shows a trend of worse prognosis in patients with cervical cancer.

Article Snippet: The sections were incubated with anti-AGR2 rabbit polyclonal antibody (Novus Biologicals, NBP2-27393, CO, USA) at a 1:250 dilution, anti-BRD7 rabbit polyclonal antibody (Novus Biologicals, NBP1-28727, CO, USA) at a 1:500 dilution, or anti-POM121 rabbit polyclonal antibody (Novus Biologicals) at a 1:500 dilution for 120 min at room temperature.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Genomic Network-Based Analysis Reveals Pancreatic Adenocarcinoma Up-Regulating Factor-Related Prognostic Markers in Cervical Carcinoma

doi: 10.3389/fonc.2018.00465

Figure Lengend Snippet: The correlation between the PAUF associated proteins expressions with clinicopathologic characteristics of cervical cancer.

Article Snippet: The sections were incubated with anti-AGR2 rabbit polyclonal antibody (Novus Biologicals, NBP2-27393, CO, USA) at a 1:250 dilution, anti-BRD7 rabbit polyclonal antibody (Novus Biologicals, NBP1-28727, CO, USA) at a 1:500 dilution, or anti-POM121 rabbit polyclonal antibody (Novus Biologicals) at a 1:500 dilution for 120 min at room temperature.

Techniques: Significance Assay

Journal: Frontiers in Oncology

Article Title: Genomic Network-Based Analysis Reveals Pancreatic Adenocarcinoma Up-Regulating Factor-Related Prognostic Markers in Cervical Carcinoma

doi: 10.3389/fonc.2018.00465

Figure Lengend Snippet: Expression of the PAUF-associated proteins AGR2, BRD7, and POM121 in patients with cervical cancer. (A) Representative examples of positive and negative expression of the AGR2, BRD7, and POM121 proteins. The scale bar represents 50 μm. (B) Correlations between expression of PAUF and PAUF-related proteins in cervical cancer tissues. AGR2 protein expression was positively correlated with POM121 expression ( r = 0.33, p < 0.001).

Article Snippet: The sections were incubated with anti-AGR2 rabbit polyclonal antibody (Novus Biologicals, NBP2-27393, CO, USA) at a 1:250 dilution, anti-BRD7 rabbit polyclonal antibody (Novus Biologicals, NBP1-28727, CO, USA) at a 1:500 dilution, or anti-POM121 rabbit polyclonal antibody (Novus Biologicals) at a 1:500 dilution for 120 min at room temperature.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Genomic Network-Based Analysis Reveals Pancreatic Adenocarcinoma Up-Regulating Factor-Related Prognostic Markers in Cervical Carcinoma

doi: 10.3389/fonc.2018.00465

Figure Lengend Snippet: Hierarchical clustering analysis for immunohistochemical expression of PAUF-associated proteins in cervical cancer patients. (A) Three cluster groups were identified in the present study. Category 1 ( n = 118, blue arrow) consists of relatively high AGR2 and POM121 expression. Category 2 ( n = 194, red arrow) consists of relatively low AGR2 and PAUF expression. Category 3 ( n = 24, green arrow) consists of relatively high PAUF and low POM121 expression. (B) Kaplan–Meier plot for overall survival according to group by hierarchical clustering. A tendency toward decreased overall survival was observed in Category 3 than in the other groups ( log -rank test, p = 0.053).

Article Snippet: The sections were incubated with anti-AGR2 rabbit polyclonal antibody (Novus Biologicals, NBP2-27393, CO, USA) at a 1:250 dilution, anti-BRD7 rabbit polyclonal antibody (Novus Biologicals, NBP1-28727, CO, USA) at a 1:500 dilution, or anti-POM121 rabbit polyclonal antibody (Novus Biologicals) at a 1:500 dilution for 120 min at room temperature.

Techniques: Immunohistochemical staining, Expressing

Journal: Frontiers in Oncology

Article Title: Genomic Network-Based Analysis Reveals Pancreatic Adenocarcinoma Up-Regulating Factor-Related Prognostic Markers in Cervical Carcinoma

doi: 10.3389/fonc.2018.00465

Figure Lengend Snippet: Kaplan–Meier plot of disease-free survival according to expression of PAUF and PAUF-associated proteins. (A,B) Patients with high AGR2 expression (AGR2 High ) and low BRD7 expression (BRD7 Low ) showed worse disease-free survival ( log -rank test, p < 0.001 and p = 0.009, respectively) than patients with low AGR2 expression (AGR2 Low ) and high BRD7 expression (BRD7 High ). (C) Patients with low POM121 expression (POM121 Low ) showed a trend of worse disease-free survival. (D–F) Patients with combined PAUF High /AGR2 High , PAUF High /BRD7 Low , or PAUF High /POM121 Low expression showed shorter disease-free survival ( log -rank test, p < 0.001, p < 0.001, and p < 0.001, respectively) than patients with the opposite combined expression profile. (G–J) Patients with combined AGR2 High /BRD7 Low , BRD7 Low /POM121 Low , or AGR2 High /BRD7 Low /POM121 Low expression showed shorter disease-free survival ( log -rank test, p < 0.001, p = 0.009, and p = 0.044, respectively) than patients with combined AGR2 Low /BRD7 High , BRD7 High /POM121 High , or AGR2 Low /BRD7 High /POM121 High expression.

Article Snippet: The sections were incubated with anti-AGR2 rabbit polyclonal antibody (Novus Biologicals, NBP2-27393, CO, USA) at a 1:250 dilution, anti-BRD7 rabbit polyclonal antibody (Novus Biologicals, NBP1-28727, CO, USA) at a 1:500 dilution, or anti-POM121 rabbit polyclonal antibody (Novus Biologicals) at a 1:500 dilution for 120 min at room temperature.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Genomic Network-Based Analysis Reveals Pancreatic Adenocarcinoma Up-Regulating Factor-Related Prognostic Markers in Cervical Carcinoma

doi: 10.3389/fonc.2018.00465

Figure Lengend Snippet: Univariate and multivariate analyses of disease free survival according to prognostic variables in cervical cancer patients ( n = 336).

Article Snippet: The sections were incubated with anti-AGR2 rabbit polyclonal antibody (Novus Biologicals, NBP2-27393, CO, USA) at a 1:250 dilution, anti-BRD7 rabbit polyclonal antibody (Novus Biologicals, NBP1-28727, CO, USA) at a 1:500 dilution, or anti-POM121 rabbit polyclonal antibody (Novus Biologicals) at a 1:500 dilution for 120 min at room temperature.

Techniques: Significance Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Associations of MACC1, AGR2, and KAI1 expression with the metastasis and prognosis in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: Expression of the patients in head and neck squamous cell carcinoma (×400 magnification). A. Negative MACC1 expression in the control normal cells. B. Positive MACC1 expression in the cytoplasm of cancer cells. C. Negative AGR2 expression in the control normal cells. D. Positive AGR2 expression in the nuclei of cancer cells. E. Positive KAI1 expression in the membrane and cytoplasm of control normal cells. F. Negative KAI1 expression in cancer cells.

Article Snippet: Subsequently placed in citrate buffer (pH 6.0) at 95°C to repair antigen for 30 min. After several washes with PBS, all slices were blocked by goat serum for 30 min, subsequently incubated with rabbit polyclonal antibody against human MACC1 (Santa Cruz Biotechnology, CA, USA) or rabbit polyclonal antibody against human AGR2 (Cell Signaling Technology, Inc., Danvers, MA, USA) or mouse monoclonal antibody against human KAI1 (Abcam Cambridge, MA, USA) at 37°C for 1 h. Finally, all slices were counterstained with hematoxylin, dehydrated, air-dried, and mounted.

Techniques: Expressing, Control, Membrane

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Associations of MACC1, AGR2, and KAI1 expression with the metastasis and prognosis in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: The associations between expression of MACC1, AGR2, and KAI1 and clinicopathological characteristics of head and neck squamous cell carcinoma (HNSCC)

Article Snippet: Subsequently placed in citrate buffer (pH 6.0) at 95°C to repair antigen for 30 min. After several washes with PBS, all slices were blocked by goat serum for 30 min, subsequently incubated with rabbit polyclonal antibody against human MACC1 (Santa Cruz Biotechnology, CA, USA) or rabbit polyclonal antibody against human AGR2 (Cell Signaling Technology, Inc., Danvers, MA, USA) or mouse monoclonal antibody against human KAI1 (Abcam Cambridge, MA, USA) at 37°C for 1 h. Finally, all slices were counterstained with hematoxylin, dehydrated, air-dried, and mounted.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Associations of MACC1, AGR2, and KAI1 expression with the metastasis and prognosis in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: Association between expression of MACC1, AGR2, and KAI1 in HNSCC

Article Snippet: Subsequently placed in citrate buffer (pH 6.0) at 95°C to repair antigen for 30 min. After several washes with PBS, all slices were blocked by goat serum for 30 min, subsequently incubated with rabbit polyclonal antibody against human MACC1 (Santa Cruz Biotechnology, CA, USA) or rabbit polyclonal antibody against human AGR2 (Cell Signaling Technology, Inc., Danvers, MA, USA) or mouse monoclonal antibody against human KAI1 (Abcam Cambridge, MA, USA) at 37°C for 1 h. Finally, all slices were counterstained with hematoxylin, dehydrated, air-dried, and mounted.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Associations of MACC1, AGR2, and KAI1 expression with the metastasis and prognosis in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: Kaplan-Meier analysis of the overall survival (OS) time of patients with head and neck squamous cell carcinoma. A. OS time of all patients in relation to MACC1 expression (log-rank =30.451, P<0.001). B. OS time of all patients in relation to AGR2 expression (log-rank =17.846, P<0.001). C. OS time of all patients in relation to KAI1 expression (log-rank =52.719, P<0.001). The green line represents positive expression of MACC1, AGR2, and KAI1 and the blue line represents negative expression of MACC1, AGR2, and KAI1.

Article Snippet: Subsequently placed in citrate buffer (pH 6.0) at 95°C to repair antigen for 30 min. After several washes with PBS, all slices were blocked by goat serum for 30 min, subsequently incubated with rabbit polyclonal antibody against human MACC1 (Santa Cruz Biotechnology, CA, USA) or rabbit polyclonal antibody against human AGR2 (Cell Signaling Technology, Inc., Danvers, MA, USA) or mouse monoclonal antibody against human KAI1 (Abcam Cambridge, MA, USA) at 37°C for 1 h. Finally, all slices were counterstained with hematoxylin, dehydrated, air-dried, and mounted.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Associations of MACC1, AGR2, and KAI1 expression with the metastasis and prognosis in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: Results of multivariate analyses of overall survival (OS) time

Article Snippet: Subsequently placed in citrate buffer (pH 6.0) at 95°C to repair antigen for 30 min. After several washes with PBS, all slices were blocked by goat serum for 30 min, subsequently incubated with rabbit polyclonal antibody against human MACC1 (Santa Cruz Biotechnology, CA, USA) or rabbit polyclonal antibody against human AGR2 (Cell Signaling Technology, Inc., Danvers, MA, USA) or mouse monoclonal antibody against human KAI1 (Abcam Cambridge, MA, USA) at 37°C for 1 h. Finally, all slices were counterstained with hematoxylin, dehydrated, air-dried, and mounted.

Techniques:

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: Identification and prognostic value of anterior gradient protein 2 expression in breast cancer based on tissue microarray.

doi: 10.1177/1010428317713392

Figure Lengend Snippet: Figure 1. The expression pattern of AGR2 in breast cancer. (a) Histogram of 69 samples from the GEO database. (b) Box plot revealing the AGR2 expression pattern. (c) Heat map of 69 samples from the GEO database. (d) Box plot of patients from TCGA database. (e) qRT-PCR validation of AGR2 expression pattern. (f) Heat map of patients from TCGA database.

Article Snippet: Briefly, the tissue microarray was deparaffinized and rehydrated for 5 min. To block endogenous peroxidase activity, the slides were maintained in hydrogen peroxide for 20 min. Then, the tissue microarray was incubated with polyclonal rabbit anti-AGR2 antibody (1:1000; 12275-1-AP; Proteintech Group, Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Biomarker Discovery

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: Identification and prognostic value of anterior gradient protein 2 expression in breast cancer based on tissue microarray.

doi: 10.1177/1010428317713392

Figure Lengend Snippet: Figure 2. Overexpression of AGR2 in breast cancer. (a) The staining results in normal breast tissues. (b) The staining results in normal breast tissue. (c) The staining results in non-TNBC tissues. (d) The staining results in TNBC tissues. (e) The staining results in non-TNBC tissues. (f) The staining results in paracancer tissues of patients included in Figure 2(e).

Article Snippet: Briefly, the tissue microarray was deparaffinized and rehydrated for 5 min. To block endogenous peroxidase activity, the slides were maintained in hydrogen peroxide for 20 min. Then, the tissue microarray was incubated with polyclonal rabbit anti-AGR2 antibody (1:1000; 12275-1-AP; Proteintech Group, Wuhan, China).

Techniques: Over Expression, Staining

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: Identification and prognostic value of anterior gradient protein 2 expression in breast cancer based on tissue microarray.

doi: 10.1177/1010428317713392

Figure Lengend Snippet: Figure 4. The prognostic value of AGR2. (a) Overall survival analysis of all patients according to AGR2 expression using Kaplan– Meier plotter. (b) Overall survival analysis of patients with grade 1 tumours according to AGR2 expression using Kaplan–Meier plotter. (c) Overall survival analysis of all patients according to AGR2 expression using tissue microarray. (d) Overall survival analysis of patients with grade 1 + 2 tumours according to AGR2 expression using tissue microarray.

Article Snippet: Briefly, the tissue microarray was deparaffinized and rehydrated for 5 min. To block endogenous peroxidase activity, the slides were maintained in hydrogen peroxide for 20 min. Then, the tissue microarray was incubated with polyclonal rabbit anti-AGR2 antibody (1:1000; 12275-1-AP; Proteintech Group, Wuhan, China).

Techniques: Expressing, Microarray

Journal: Oncology reports

Article Title: Establishment and characterization of a novel human cholangiocarcinoma cell line with high metastatic activity.

doi: 10.3892/or.2016.4974

Figure Lengend Snippet: Figure 6. AGR2 and KiSS-1 expression in CCA patient tissues. Immunohistochemistry of AGR2 and KiSS-1 was performed in 32 cases of intrahepatic mass-forming CCA patient tissues with different tumor staging (A). The immunohistochemical (IHC) indices were determined in normal bile duct (NBD, n=30), CCA stage III (n=10), CCA stage IVA (n=11) and CCA stage IVB (n=11). Normal bile duct epithelia from adjacent non-tumorous tissues were assessed. Semi-quantitative analysis of AGR2 and KiSS-1 expression levels presented as IHC indices are shown in (B); *P<0.05, ***P<0.001.

Article Snippet: AGR2 and KiSS-1 immunohistochemistry was performed in 32 intrahepatic mass-forming CCA patient tissues using 1:500 rabbit polyclonal anti-AGR2 antibody (Abcam, Cambridge, MA, USA) and 1:50 rabbit polyclonal anti-KiSS-1 antibody (Santa Cruz biotechnology, Dallas, TX, USA) at room temperature, overnight.

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining

Journal: Oncotarget

Article Title: Anterior gradient protein 2 expression in high grade head and neck squamous cell carcinoma correlated with cancer stem cell and epithelial mesenchymal transition

doi:

Figure Lengend Snippet: (A) AGR2 DNA copy number from Peng's dataset and (B) mRNA expression from Ginos's dataset (as log2 median –centered ratio) for head neck cancer versus normal counterpart shown as raw data. (C) AGR2 DNA copy number of TCGA head neck cancer as shown as raw data. (D) Representative immunohistochemistry staining of AGR2 in oral mucosa as well as in HNSCC tissue with quantification in (E, One way ANOVA , P <0.001). Dys, dysplasia; SCC, squamous cell carcinoma; ***, P <0.001.

Article Snippet: After being incubated with 3% hydrogen superoxide for 20 minutes to quench endogenous peroxidase activity and 10% normal goat serum to block non-specific binding, the sections were incubated overnight at 4 °C with polyclonal rabbit anti-human AGR2 (Cell Signaling Technology, 1:200), Survivin (Cell Signaling Technology, 1:200), Cyclin D1 (Epitomics, 1:200), ALDH1 (Proteintech gruop, 1:100), (Epitomics, 1:200), OCT4 (Proteintech gruop, 1:100), Slug (Cell Signaling Technology, 1:200).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Oncotarget

Article Title: Anterior gradient protein 2 expression in high grade head and neck squamous cell carcinoma correlated with cancer stem cell and epithelial mesenchymal transition

doi:

Figure Lengend Snippet: (A) Representative hematoxylin-eosin staining (HE, left) and immunohistochemical (IHC) staining (right) of AGR2 in human oral cancer tissues with different grades (I-III) (Scale bars =50um); (B) Quantitative analysis of histoscore of AGR2 expression in normal oral mucosa, oral epithelial dysplasia and human HNSCC. AGR2 levels in HNSCC and/or oral epithelial dysplasia was significant higher when compared with normal oral mucosa (One way ANOVA ,P < 0.001); (C) The expression of AGR2 was correlated with T category of human oral cancer(One way ANOVA, P < 0.001); (D) The expression of AGR2 was correlated with lymph node metastasis of human HNSCC ( t test, P < 0.01). **, P <0.01; ***, P <0.001. Scale bars=50um

Article Snippet: After being incubated with 3% hydrogen superoxide for 20 minutes to quench endogenous peroxidase activity and 10% normal goat serum to block non-specific binding, the sections were incubated overnight at 4 °C with polyclonal rabbit anti-human AGR2 (Cell Signaling Technology, 1:200), Survivin (Cell Signaling Technology, 1:200), Cyclin D1 (Epitomics, 1:200), ALDH1 (Proteintech gruop, 1:100), (Epitomics, 1:200), OCT4 (Proteintech gruop, 1:100), Slug (Cell Signaling Technology, 1:200).

Techniques: Staining, Immunohistochemical staining, Immunohistochemistry, Expressing

Journal: Oncotarget

Article Title: Anterior gradient protein 2 expression in high grade head and neck squamous cell carcinoma correlated with cancer stem cell and epithelial mesenchymal transition

doi:

Figure Lengend Snippet: (A) Representative HE staining (left) and immunohistochemical staining of recurrence HNSCC (right) after radiotherapy; (B) Representative HE staining (left) and immunohistochemical staining (right) of recurrence HNSCC after combined cisplatin, docetaxel, and fluorouracil (TPF) chemotherapy; (C) Representative HE staining (left) and immunohistochemical (right) staining of original HNSCC and lymph node metastasis;(D) Quantitative of immunohistochemistry satinning using digital scanner and histoscore of AGR2 expression after radiotherapy was significantly higher than original HNSCC ( t test, P < 0.05). (E) The expression level of AGR2 in recurrence after TPF chemotherapy was significantly higher than original HNSCC (paired t test, P < 0.001); (F) There was no significant difference between the expression level of AGR2 in original HNSCC and lymph node metastasis( t test, P>0.05 ). Scale bars=50um

Article Snippet: After being incubated with 3% hydrogen superoxide for 20 minutes to quench endogenous peroxidase activity and 10% normal goat serum to block non-specific binding, the sections were incubated overnight at 4 °C with polyclonal rabbit anti-human AGR2 (Cell Signaling Technology, 1:200), Survivin (Cell Signaling Technology, 1:200), Cyclin D1 (Epitomics, 1:200), ALDH1 (Proteintech gruop, 1:100), (Epitomics, 1:200), OCT4 (Proteintech gruop, 1:100), Slug (Cell Signaling Technology, 1:200).

Techniques: Staining, Immunohistochemical staining, Immunohistochemistry, Expressing

Journal: Oncotarget

Article Title: Anterior gradient protein 2 expression in high grade head and neck squamous cell carcinoma correlated with cancer stem cell and epithelial mesenchymal transition

doi:

Figure Lengend Snippet: (A) Representative immunohistochemical staining (IHC) of AGR2, Survivin, Cyclin D1, ALDH1, Oct4 and Slug in human oral cancer tissue (right) compared with normal oral mucosa (left) (Scale bars =50um); (B) Hierarchical clustering present the protein expression correlation of AGR2, Survivin, Cyclin D1, ALDH1, Sox2, Oct4 and Slug in human HNSCC tissue array.

Article Snippet: After being incubated with 3% hydrogen superoxide for 20 minutes to quench endogenous peroxidase activity and 10% normal goat serum to block non-specific binding, the sections were incubated overnight at 4 °C with polyclonal rabbit anti-human AGR2 (Cell Signaling Technology, 1:200), Survivin (Cell Signaling Technology, 1:200), Cyclin D1 (Epitomics, 1:200), ALDH1 (Proteintech gruop, 1:100), (Epitomics, 1:200), OCT4 (Proteintech gruop, 1:100), Slug (Cell Signaling Technology, 1:200).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Oncotarget

Article Title: Anterior gradient protein 2 expression in high grade head and neck squamous cell carcinoma correlated with cancer stem cell and epithelial mesenchymal transition

doi:

Figure Lengend Snippet: (A) Annexin V-FITC/PI dual labeling assay showed AGR2 siRNA enhance apoptosis in CAL27 cell lines by using flow cytometry; (B) The morphologic changes of CAL27 cell lines transfected with AGR2 siRNA was observed by fluorescence microscopy with DAPI staining; (C) Knock down of AGR2 in CAL27 cell line reduced anchor dependent colony formation; (D) Knock down of AGR2 in CAL27 cell line reduced sphere formation; Scale bar=100μm;(E) Western blot analysis revealed that the protein level of Survivin, Cyclin D1, Bcl2, Bcl2l1, Slug, Snail, Nanog, Sox2 and OCT4 were reduced in different degrees after AGR2 konck down for 48h. Quantification is performed using Image J by pixel analysis of band by normalized of β-actin as a loading control. Neg siRNA, negative siRNA,si AGR2, AGR2 siRNA.

Article Snippet: After being incubated with 3% hydrogen superoxide for 20 minutes to quench endogenous peroxidase activity and 10% normal goat serum to block non-specific binding, the sections were incubated overnight at 4 °C with polyclonal rabbit anti-human AGR2 (Cell Signaling Technology, 1:200), Survivin (Cell Signaling Technology, 1:200), Cyclin D1 (Epitomics, 1:200), ALDH1 (Proteintech gruop, 1:100), (Epitomics, 1:200), OCT4 (Proteintech gruop, 1:100), Slug (Cell Signaling Technology, 1:200).

Techniques: Labeling, Flow Cytometry, Transfection, Fluorescence, Microscopy, Staining, Knockdown, Western Blot, Control

Journal: Oncotarget

Article Title: Anterior gradient protein 2 expression in high grade head and neck squamous cell carcinoma correlated with cancer stem cell and epithelial mesenchymal transition

doi:

Figure Lengend Snippet: (A) Immunohistochemistry of AGR2 in tongue of the wild type (WT) mice, the tumor of Pten conditional knock out mice, Tgfbr1 conditional knock out mice and Pten/Tgfbr1 conditional knock out mice (Scale bar+25μm); (B) Quantitative of histoscore of AGR2 in wild type mice, Pten conditional knock out mice, Tgfbr1 conditional knock out mice and Pten/Tgfbr1 conditional knock out mice. Expression of AGR2 in Tgfbr1 conditional knock out mice and Pten/Tgfbr1 conditional knock out mice was significantly higher than the wild type mice ( P < 0.001); (C) Quantitative Real-time PCR revealed the mRNA level of AGR2 in Tgfbr1 conditional knock out mice and Pten/Tgfbr1 conditional knock out mice was significantly increased when compared with the wild type mice; (D) Western blot analysis of AGR2 48h after knocking down PTEN, TGFBR1, and combined TGFBR1/PTEN by using siRNA; (E) Quantitative analysis showed the protein level of AGR2 in TGFBR1 siRNA group and TGFBR1/PTEN combined siRNA group were significantly higher than the control group ( P < 0.001). Mean±SEM, ***, P <0.001.

Article Snippet: After being incubated with 3% hydrogen superoxide for 20 minutes to quench endogenous peroxidase activity and 10% normal goat serum to block non-specific binding, the sections were incubated overnight at 4 °C with polyclonal rabbit anti-human AGR2 (Cell Signaling Technology, 1:200), Survivin (Cell Signaling Technology, 1:200), Cyclin D1 (Epitomics, 1:200), ALDH1 (Proteintech gruop, 1:100), (Epitomics, 1:200), OCT4 (Proteintech gruop, 1:100), Slug (Cell Signaling Technology, 1:200).

Techniques: Immunohistochemistry, Knock-Out, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Control